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Delta software for nmrsd. The appreciable binding of the double mutant Su(H) LL/AA to Hairless observed in vitro was uncovered in our in vivo experiment with a repression of Notch activity similar to wild type Su(H) ( panel 8).Finally, these results were confirmed by a phenotypic analysis of adult flies, in which the Su(H) mutant constructs and Hairless were coexpressed during eye development. As shown in, and consistent with our previous results, ectopic induction of all three Su(H) mutants in the eye imaginal discs effects eye morphology indistinguishable from wild-type Su(H), but the triple mutants Su(H) LLL/AAA and Su(H) LLF/AAA retain little to no binding of Hairless in vivo. Ectopic expression of Su(H) LLF/AAA with Hairless was less effective ( panel 9), perhaps reflecting residual binding to Hairless and/or potentially subtle defects in activator complex formation, which our other assays were not sensitive enough to detect. As shown in (panel 10), ectopic expression of the triple mutant Su(H) LLL/AAA with Hairless resulted in normal expression of the vg BE -lacZ reporter and the wing disc was similar to the control, suggesting an almost complete loss of Hairless binding by the mutant.